EIF4A2 Antibody
| Code | Size | Price |
|---|
| PSI-29-278-100ul | 100ul | £662.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Canine (dog)
- Human
- Mouse
- Rat
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
For short periods of storage (days) store at 4˚C. For longer periods of storage, store EIF4A2 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Documents
Further Information
Additional Names:
EIF4A2, DDX2B, EIF4A, EIF4F, BM-010, eIF4A-II, eIF-4A-II
Application Note:
EIF4A2 antibody can be used for detection of EIF4A2 by ELISA at 1:312500. EIF4A2 antibody can be used for detection of EIF4A2 by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Background:
EIF4A2 contains 1 helicase C-terminal domain and 1 helicase ATP-binding domain. It belongs to the DEAD box helicase family. eIF4A subfamily. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5' untranslated region of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.
Background References:
- Sarkar, M., (2005) FEBS Lett. 579 (16), 3449-3460.
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human EIF4A2.
NCBI Gene ID #:
1974
NCBI Official Name:
eukaryotic translation initiation factor 4A2
NCBI Official Symbol:
EIF4A2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
45 kDa
Protein Accession #:
NP_001958
Protein GI Number:
83700235
Purification:
Antibody is purified by peptide affinity chromatography method.
Research Area:
RNA Binding
Swissprot #:
Q14240
User NOte:
Optimal dilutions for each application to be determined by the researcher.