HNRPH1 Antibody

ProSci
Product Code: PSI-27-173
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-27-173-100ul100ul£662.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
For short periods of storage (days) store at 4˚C. For longer periods of storage, store HNRPH1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.

Images

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Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.
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Antibody used in WB on HeLa S3, MCF7, K562 at 1:4000 (Lane 1: 20ug HeLa S3 lysate Lane 2: 20ug MCF7 lysate Lane 3: 20ug K562 lysate ).
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Antibody used in IF on MCF7 cells at 1:200.
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Antibody used in IP on K562 at 1:4000 (Lane 1: 5% InputLane 2: 5% SupLane 3: Normal IgGLane 4: hn-RNPH1 ppt. k562 sample ).
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Antibody used in WB on Human MCF7 at 1 ug/ml.
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Antibody used in WB on Human Jurkat 1 ug/ml.
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Antibody used in WB on Human 721_B cells at 1 ug/ml.

Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.
Antibody used in WB on HeLa S3, MCF7, K562 at 1:4000 (Lane 1: 20ug HeLa S3 lysate Lane 2: 20ug MCF7 lysate Lane 3: 20ug K562 lysate ).
Antibody used in IF on MCF7 cells at 1:200.
Antibody used in IP on K562 at 1:4000 (Lane 1: 5% InputLane 2: 5% SupLane 3: Normal IgGLane 4: hn-RNPH1 ppt. k562 sample ).
Antibody used in WB on Human MCF7 at 1 ug/ml.
Antibody used in WB on Human Jurkat 1 ug/ml.
Antibody used in WB on Human 721_B cells at 1 ug/ml.

Further Information

Additional Names:
HNRPH1, DKFZp686A15170, HNRPH, hnRNPH, HNRPH1
Application Note:
HNRPH1 antibody can be used for detection of HNRPH1 by ELISA at 1:62500. HNRPH1 antibody can be used for detection of HNRPH1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Background:
HNRPH1 belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. This protein has three repeats of quasi-RRM domains that bind to RNAs. It is very similar to the family member HNRPF. This gene is thought to be potentially involved in hereditary lymphedema type I phenotype.This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has three repeats of quasi-RRM domains that bind to RNAs. It is very similar to the family member HNRPF. This gene is thought to be potentially involved in hereditary lymphedema type I phenotype. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Background References:
  • Olsen, J.V., (2006) Cell 127 (3), 635-648.
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human HNRPH1.
NCBI Gene ID #:
3187
NCBI Official Name:
heterogeneous nuclear ribonucleoprotein H1 (H)
NCBI Official Symbol:
HNRNPH1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
49 kDa
Protein Accession #:
NP_005511
Protein GI Number:
5031753
Purification:
Antibody is purified by peptide affinity chromatography method.
Research Area:
RNA Binding
Swissprot #:
P31943
User NOte:
Optimal dilutions for each application to be determined by the researcher.