SF3B1 Antibody

Contact us
ProSci
Product Code: PSI-29-408
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-29-408-100ul100ul£609.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Canine (dog)
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
For short periods of storage (days) store at 4˚C. For longer periods of storage, store SF3B1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.

Images

1 / 1
Antibody used in WB on Human Jurkat cells at 0.3125 ug/ml.
❮❯

Antibody used in WB on Human Jurkat cells at 0.3125 ug/ml.

Documents

Further Information

Additional Names:
SF3B1, MDS, PRP10, Hsh155, PRPF10, SAP155, SF3b155
Application Note:
SF3B1 antibody can be used for detection of SF3B1 by ELISA at 1:312500. SF3B1 antibody can be used for detection of SF3B1 by western blot at 0.3125 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Background:
SF3B1 is subunit 1 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. The carboxy-terminal two-thirds of subunit 1 have 22 non-identical, tandem HEAT repeats that form rod-like, helical structures.
Background References:
  • Strausberg, R.L., Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002).
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SF3B1.
NCBI Gene ID #:
23451
NCBI Official Name:
splicing factor 3b, subunit 1, 155kDa
NCBI Official Symbol:
SF3B1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
87 kDa, 146 kDa
Protein Accession #:
AAH56155
Protein GI Number:
33525215
Purification:
Antibody is purified by protein A chromatography method.
Research Area:
RNA Binding
Swissprot #:
O75533
User NOte:
Optimal dilutions for each application to be determined by the researcher.