MGAT1 Antibody

ProSci
Product Code: PSI-42-645
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-42-645-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human MGAT1 with C-terminal MYC tag probed with 42-645 (1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with 42-645 (1mg/ml) in Lane B. Primary

HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human MGAT1 with C-terminal MYC tag probed with 42-645 (1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with 42-645 (1mg/ml) in Lane B. Primary

Further Information

Additional Names:
MGAT1, mannosyl (alpha-1,3-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase, GLCNAC-TI, GLCT1, GLYT1, GNT-1, GNT-I, MGAT, N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase, OTTHUMP00000161546
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:In transfected HEK293 transiently expressing full-length Human MGAT1 (myc and DYKDDDDK tagged), a band of approx. 55kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-myc tag antibody. Recommended concentration, 0.2-1ug/ml.Purchase the +ve control! The transfected lysate used to QC this product is available for sale. Please email sales@everestbiotech.com to request datasheet, pricing and delivery information.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-QVEKVRTNDR
NCBI Gene ID #:
4245
NCBI Official Name:
mannosyl (alpha-1,3-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase
NCBI Official Symbol:
MGAT1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_002397.2
Protein GI Number:
167857778
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Signal Transduction
SPECIFICITY:
Reported variants represent identical protein: NP_002397.2, NP_001108090.1, NP_001108091.1, NP_001108092.1, NP_001108089.1.
Swissprot #:
P26572