MEC Antibody

ProSci
Product Code: PSI-38-268
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-38-268-0.1mg0.1mg£524.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
The lyophilized antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-80˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repea

Images

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To detect hMEC by sandwich ELISA (using 100ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci?s Biotinylated Anti-Human MEC (38-279) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMEC.
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This antibody stained CACO-2 cells.  The primary antibody was incubated at 3.0 ug/ml overnight at 4˚C followed by a fluorescent labeled secondary antibody.  Optimal concentrations and conditions may vary.  Information courtesy of the Cell Profiling group, SciLifeLab Stockholm.

To detect hMEC by sandwich ELISA (using 100ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci?s Biotinylated Anti-Human MEC (38-279) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMEC.
This antibody stained CACO-2 cells.  The primary antibody was incubated at 3.0 ug/ml overnight at 4˚C followed by a fluorescent labeled secondary antibody.  Optimal concentrations and conditions may vary.  Information courtesy of the Cell Profiling group, SciLifeLab Stockholm.

Further Information

Additional Names:
MEC, CCK1, SCYA28, C-C motif chemokine 28, Mucosae-associated epithelial chemokine, MEC
Application Note:
ELISA:
Sandwich:
To detect hMEC by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci?s Biotinylated Anti-Human MEC (38-279) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMEC.

Western Blot:

To detect hMEC by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMEC is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Produced from sera of rabbits immunized with highly pure recombinant Human MEC. Human MEC specific antibody was purified by affinity chromatography employing an immobilized Human MEC matrix.
NCBI Gene ID #:
56477
NCBI Official Name:
chemokine (C-C motif) ligand 28
NCBI Official Symbol:
CCL28
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
Q9NRJ3
Protein GI Number:
12230650
Research Area:
Antibody Pairs
Swissprot #:
Q9NRJ3
User NOte:
Centrifuge vial prior to opening.