RET Monoclonal Antibody
| Code | Size | Price |
|---|
| PSI-32-211-0.1mL | 0.1mL | £560.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG1
Antibody Clonality: Monoclonal
Antibody Clone: 6E4C4
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
RET monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
CDHF12, CDHR16, PTC, RET51, Cadherin family member 12PTC, MTC1, HSCR1, MEN2A, MEN2B, CDHF12, RET-ELE1
Application Note:
Western Blot:1:500 - 1:2,000
IHC(P):1:500 - 1:2,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
IHC(P):1:500 - 1:2,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
Background:
RET (ret proto-oncogene) is a member of the cadherin superfamily and a receptor tyrosine kinase, which are cell-surface molecules that transduce signals for cell growth and differentiation. It can undergo oncogenic activation in vivo and in vitro by cytogenetic rearrangement. Ligands that bind the Ret receptor include the glial cell line-derived neurotropic factor (GDNF) and its congeners neurturin, persephin and artemin. Alterations in the corresponding Ret gene are associated with diseases including papillary thyroid carcinoma, multiple endocrine neoplasia (type 2A and 2B), familial medullary thyroid carcinoma and a congenital developmental disorder known as Hirschsprung? disease. The Tyr905 residue located in the Ret kinase domain plays a crucial role in Ret catalytic and biological activity. Substitution of Phe for Tyr905 dramatically inhibits Ret autophosphorylation activity.
Background References:
- Young HM. Anderson RB. Anderson CR. Auton Neurosci. 2004, May 31,112(1-2):1-14.
- Myers SM. Mulligan LM. Cancer Res. 2004, Jul 1,64(13):4453-63.
Buffer:
Ascitic fluid containing 0.03% sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Purified truncated recombinant RET (aa896-1063)-GST fusion protein is expressed in E. Coli strain BL21 (DE3).
NCBI Gene ID #:
5979
NCBI Official Name:
ret proto-oncogene
NCBI Official Symbol:
RET
NCBI Organism:
Homo sapiens
Protein Accession #:
P07949
Protein GI Number:
547807
Research Area:
Signal Transduction
Swissprot #:
P07949
User NOte:
Optimal dilutions for each application to be determined by the researcher.