CIRBP Antibody

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ProSci
Product Code: PSI-42-632
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-42-632-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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42-632 (0.05ug/ml) staining of Mouse Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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42-632 (0.05ug/ml) staining of Mouse Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

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Further Information

Additional Names:
CIRBP, cold inducible RNA binding protein, CIRP, Cold-inducible RNA-binding protein, cold inducible RNA-binding protein, glycine-rich RNA binding protein
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 18kDa band observed in Mouse and Rat Testis lysates (calculated MW of 18.6kDa according to Mouse NP_031731.1 and Rat NP_112409.2). Recommended concentration: 0.05-0.2ug/ml. An additional band of unknown identity was also consistently observed at 23kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Human, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-QAGKSSDNRSR
NCBI Gene ID #:
1153
NCBI Official Name:
cold inducible RNA binding protein
NCBI Official Symbol:
CIRBP
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_001271.1
Protein GI Number:
4502847
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
SPECIFICITY:
.
Swissprot #:
Q14011