CD10 Monoclonal Antibody

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ProSci
Product Code: PSI-32-127
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-32-127-0.1mg0.1mg£524.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: IgG2b
Antibody Clonality: Monoclonal
Antibody Clone: 2A1H5E1
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
Storage:
CD10 monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Immunohistochemical analysis of paraffin - embedded human breast ductal myoepithelium showing cytoplasmic localization using CD10 antibody with DAB staining.
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Immunohistochemical analysis of paraffin - embedded human breast ductal myoepithelium showing cytoplasmic localization using CD10 antibody with DAB staining.

Documents

Further Information

Additional Names:
EPN, Atriopeptidase, CALLA, NEP, SFE, CD10
Application Note:
IHC(P):Dilution 1:100 - 1:200
IHC(F):Dilution 1:200 - 1:400
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
Background:
CD10 is a 100 kDa glycoprotein, also designated Common Acute Lymphocytic Leukemia Antigen (CALLA). It is a cell surface enzyme with neutral metalloendopeptidase activity which inactivates a variety of biologically active peptides. CD10 is expressed on the cells of lymphoblastic, Burkitt?s, and follicular germinal center lymphomas, and on cells from patients with chronic myelocytic leukemia (CML). It is also expressed on the surface of normal early lymphoid progenitor cells, immature B cells within adult bone marrow and germinal center B cells within lymphoid tissue. CD10 is also present on breast myoepithelial cells, bile canaliculi, fibroblasts, with especially high expression on the brush border of kidney and gut epithelial cells.
Background References:
  • Sato Y,et.al. Journal of Gastroenterology,1996 Feb.31(1):12-7.
  • Inukai T,et.al. British Journal of Haematology,1995,89(3):623-6.
Buffer:
Purified antibody in PBS containing 0.03% sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Ni-NTA purified recombinant human CD-10 expressed in E. Coli strain BL21 (DE3).
NCBI Gene ID #:
4311
NCBI Official Name:
membrane metallo-endopeptidase
NCBI Official Symbol:
MME
NCBI Organism:
Homo sapiens
Protein Accession #:
P08473
Protein GI Number:
128062
Purification:
Antibody is purified by protein G affinity chromatography.
Research Area:
Signal Transduction
Swissprot #:
P08473
User NOte:
Optimal dilutions for each application to be determined by the researcher.