ADAM12 Antibody
| Code | Size | Price |
|---|
| PSI-45-001-0.1mg | 0.1mg | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.
Documents
Further Information
Additional Names:
ADAM12, MCMP, MLTN, MLTNA, MCMPMltna, a disintegrin and metalloproteinase domain 12 (meltrin alpha), meltrin alpha, A disintegrin and metalloproteinase domain 12 (Meltrin-alpha, mouse, homolog of), ADAM metallopeptidase domain 12, RP11-295J3.5
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Immunohistochemistry:In paraffin embedded Human Prostate shows strong cytoplasm staining of a group of secretory epithelial cells. Recommended concentration: 3-5ug/ml. Paraffin embedded Human Placenta. Recommended concentration: 3.75ug/ml.Immunofluorescence: Strong expression of the protein seen in the cytoplasm and plasma membranes of A431 cells. Recommended concentration: 10ug/ml.
Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.
Background References:
- Galliano MF, Huet C, Frygelius J, Polgren A, Wewer UM, Engvall E. Binding of ADAM12, a marker of skeletal muscle regeneration, to the muscle-specific actin-binding protein, alpha -actinin-2, is required for myoblast fusion. J Biol Chem. 2000 May 5;275(18):13933-9.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: AARPLPVSPARALC
NCBI Gene ID #:
8038
NCBI Official Name:
ADAM metallopeptidase domain 12
NCBI Official Symbol:
ADAM12
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_003465.3, NP_067673.2
Protein GI Number:
73747885
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Homeostasis
SPECIFICITY:
This antibody is expected to recognise both the longer membrane-bound form of human ADAM12 (NP_003465.3) and the shorter soluble ADAM12 splice isoform (NP_067673.2). This antibody does not cross-react with other ADAMS.
Swissprot #:
O43184