Alpha2-antiplasmin Antibody
| Code | Size | Price |
|---|
| PSI-45-243-0.1mg | 0.1mg | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme Immunoassay (EIA)
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.
Documents
Further Information
Additional Names:
SERPINF2, serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 2, A2AP, AAP, ALPHA-2-PI, API, PLI, alpha-2-plasmin inhibitor, serine (or cysteine) proteinase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 2
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx. 70kDa band observed in Human Duodenum and Ileum lysates (calculated MW of 54.6kDa according to NP_000925.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Lee et al, Blood. 2004 May 15;103(10):3783-8.; PMID: 14751930). Recommended concentration: 0.3-1ug/ml.
An additional band of unknown identity was also consistently observed at 26kDa. This band was successfully blocked by incubation with the immunising peptide.Immunohistochemistry:In paraffin embedded Human Liver shows spotty cytoplasm staining in hepatocytes. Recommended concentration, 3-5ug/ml.Enzyme immunoassay: This product has been successfully used in sandwich-type ELISA as the capturing antibody.
Background References:
- Matsuno H, Okada K, Ueshima S, Matsuo O, Kozawa O. Alpha2-antiplasmin plays a significant role in acute pulmonary embolism. J Thromb Haemost. 2003 Aug;1(8):1734-9
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-KDFLQSLKGFPRGDK
NCBI Gene ID #:
5345
NCBI Official Name:
serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 2
NCBI Official Symbol:
SERPINF2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_000925.2, NP_001159393.1
Protein GI Number:
115583663
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
This antibody is expected to recognise both reported isoforms (NP_000925.2; NP_001159393.1). Reported variants represent identical protein (NP_000925.2; NP_001159392.1).
Swissprot #:
P08697