EIF2AK1 Antibody
| Code | Size | Price |
|---|
| PSI-25-766-100ul | 100ul | £662.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Rat
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
For short periods of storage (days) store at 4˚C. For longer periods of storage, store EIF2AK1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Documents
Further Information
Additional Names:
EIF2AK1, HRI, KIAA1369, HCR
Application Note:
EIF2AK1 antibody can be used for detection of EIF2AK1 by ELISA at 1:62500. EIF2AK1 antibody can be used for detection of EIF2AK1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Background:
EIF2AK1 acts at the level of translation initiation to downregulate protein synthesis in response to stress. The protein is a kinase that can be inactivated by hemin. Two transcript variants encoding different isoforms have been found for this gene.The HRI gene is localized to 7p22 where its 3' end slightly overlaps the 3' end of the gene JTV1. The two genes are transcribed from opposite strands. Studies in rat and rabbit suggest that the HRI gene product phosphorylates the alpha subunit of eukaryotic initiation factor 2. Its kinase activity is induced by low levels of heme and inhibited by the presence of heme. Sequence Note: The sequence AF181071.1 is a chimeric mRNA clone. Only the heme-regulated initiation factor 2-alpha kinase region was propagated into this RefSeq record.
Background References:
- Olsen, J.V., (2006) Cell 127 (3), 635-648.
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human EIF2AK1.
NCBI Gene ID #:
27102
NCBI Official Name:
eukaryotic translation initiation factor 2-alpha kinase 1
NCBI Official Symbol:
EIF2AK1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
71 kDa
Protein Accession #:
NP_055228
Protein GI Number:
11125768
Purification:
Antibody is purified by peptide affinity chromatography method.
Research Area:
Signal Transduction
Swissprot #:
Q9BQI3
User NOte:
Optimal dilutions for each application to be determined by the researcher.