MRPS15 Antibody
| Code | Size | Price |
|---|
| PSI-27-821-100ul | 100ul | £662.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Storage:
For short periods of storage (days) store at 4˚C. For longer periods of storage, store MRPS15 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Documents
Further Information
Additional Names:
MRPS15, DC37, FLJ11564, MPR-S15, RPMS15, S15mt
Application Note:
MRPS15 antibody can be used for detection of MRPS15 by ELISA at 1:12500. MRPS15 antibody can be used for detection of MRPS15 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Background:
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. MRPS15 is a 28S subunit protein that belongs to the ribosomal protein S15P family.Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S15P family. The encoded protein is more than two times the size of its E. coli counterpart, with the 12S rRNA binding sites conserved. Between human and mouse, the encoded protein is the least conserved among small subunit ribosomal proteins. Pseudogenes corresponding to this gene are found on chromosomes 15q and 19q.
Background References:
- Zhang, Z. (2003) Genomics 81 (5), 468-480.
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human MRPS15.
NCBI Gene ID #:
64960
NCBI Official Name:
mitochondrial ribosomal protein S15
NCBI Official Symbol:
MRPS15
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
30 kDa
Protein Accession #:
NP_112570
Protein GI Number:
16554611
Purification:
Antibody is purified by peptide affinity chromatography method.
Research Area:
Signal Transduction
Swissprot #:
P82914
User NOte:
Optimal dilutions for each application to be determined by the researcher.