ATF4 Antibody

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ProSci
Product Code: PSI-45-013
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-013-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-013 (0.3ug/ml) staining of lysate of cell line K562 (35ug protein in RIPA buffer). Detected by chemiluminescence.
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45-013 (0.3ug/ml) staining of lysate of cell line K562 (35ug protein in RIPA buffer). Detected by chemiluminescence.

Documents

Further Information

Additional Names:
ATF4, activating transcription factor 4 (tax-responsive enhancer element B67), CREB2, TXREB, CREB-2, TAXREB67, OTTHUMP00000199130, activating transcription factor 4, cAMP response element-binding protein 2
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 37kDa band observed in K562 cell lysates (calculated MW of 38.6kDa according to NP_001666.2 and of NP_877962.1). Recommended concentration: 0.3-1ug/ml. Primary incubation 1 hour at room temperature.
Background References:
  • Tsujimoto A, Nyunoya H, Morita T, Sato T, Shimotohno K. Isolation of cDNAs for DNA-binding proteins which specifically bind to a tax-responsive enhancer element in the long terminal repeat of human T-cell leukemia virus type I. J Virol 1991 Mar;65(3):1420-6
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Pig
Immunogen:
The immunogen for this antibody is: C-EEVRKARGKKRVP
NCBI Gene ID #:
468
NCBI Official Name:
activating transcription factor 4
NCBI Official Symbol:
ATF4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_001666.2, NP_877962.1
Protein GI Number:
33469976
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Signal Transduction ,p53 Network ,Cancer
SPECIFICITY:
This antibody is expected to recognize both reported isoforms (NP_001666.2; NP_877962.1).
Swissprot #:
P18848