CD244 Antibody

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ProSci
Product Code: PSI-42-220
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-42-220-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunoprecipitation (IP)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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42-220 (2ug/ml) staining of IP from a lysate of Human Natural Killer cells with (+) or without (-) pervanadate treatment of the cells. Primary incubation was 1 hour. Detected by chemiluminescence. Data kindly provided by Dr Nicholas G Clarkson, Sir Willi
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42-220 (2ug/ml) staining of IP from a lysate of Human Natural Killer cells with (+) or without (-) pervanadate treatment of the cells. Primary incubation was 1 hour. Detected by chemiluminescence. Data kindly provided by Dr Nicholas G Clarkson, Sir Willi

Documents

Further Information

Additional Names:
CD244, CD244 molecule, natural killer cell receptor 2B4, 2B4, NAIL, NKR2B4, Nmrk, SLAMF4, CD244 natural killer cell receptor 2B4, NK cell activation inducing ligand NAIL, natural killer cell receptor 2B4
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 75kDa band observed in Human Natural Killer (NK) cell lysates after immunoprecipitation with mouse monoclonal antibody C1.7 coupled to Protein G agarose (calculated MW of 41.1kDa according to NP_057466.1). We do note that CD244 is a glycoprotein which makes a higher size band to be expected. Recommended concentration: 1-3ug/ml.
Background References:
  • Tangye SG, Lazetic S, Woollatt E, Sutherland GR, Lanier LL, Phillips JH Cutting edge: human 2B4, an activating NK cell receptor, recruits the protein tyrosine phosphatase SHP-2 and the adaptor signaling protein SAP. J Immunol. 1999 Jun 15;162(12):6981-5.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-EFLTIpYEDVKD
NCBI Gene ID #:
51744
NCBI Official Name:
CD244 molecule, natural killer cell receptor 2B4
NCBI Official Symbol:
CD244
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_057466.1
Protein GI Number:
7706529
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Stem Cell
Swissprot #:
Q9BZW8