COX4I1 Antibody

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ProSci
Product Code: PSI-45-424
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-424-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-424 (0.01ug/ml) staining of human muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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45-424 (0.01ug/ml) staining of human muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Documents

Further Information

Additional Names:
COX4I1, cytochrome c oxidase subunit IV isoform 1 , COX4, COXIV, MGC72016
Application Note:
Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 18kDa band observed in human skeletal muscle lysates (calculated MW of 19.6kDa according to NP_001852.1). Recommended concentration: 0.01-0.03ug/ml.Immunohistochemistry:Paraffin embedded Human Thyroid and Testis. Recommended concentration: 2.5ug/ml.
Background References:
  • Fukuda R, Zhang H, Kim JW, Shimoda L, Dang CV, Semenza GL. HIF-1 regulates cytochrome oxidase subunits to optimize efficiency of respiration in hypoxic cells. Cell. 2007 Apr 6;129(1):111-22.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-QGLASKWDYEKNE
NCBI Gene ID #:
1327; 84701
NCBI Official Name:
cytochrome c oxidase subunit IV isoform 1
NCBI Official Symbol:
COX4I1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_001852.1, NP_001305726.1
Protein GI Number:
4502981
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
This antibody is expected to recognize reported isoforms 1 and 4 (NP_001852.1; NP_001305726.1). Reported variants represent identical protein: NP_001305715.1, NP_001852.1. This product may cross-react to COX4I2 (GeneID 84701).
Swissprot #:
P13073