EBF1 Antibody

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ProSci
Product Code: PSI-46-935
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-935-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunoprecipitation (IP)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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46-935 (0.3ug/ml) staining of Human Adipose lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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46-935 (0.3ug/ml) staining of Human Adipose lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

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Further Information

Additional Names:
EBF1, early B-cell factor 1 , COE1, EBF, O/E-1, OLF1 , Collier, Olf and EBF transcription factor 1, early B-cell factor olfactory neuronal transcription factor 1
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx. 75kDa band observed in Human Adipose lysates (calculated MW of 64.5kDa according to NP_076870.1). Recommended concentration: 0.3-0.1ug/ml.Immunoprecipitation: Anonymous customer used this product successfully in IP from induced 3T3-L1 expressing FLAG-EBF1.
Background References:
  • Lagergren A, Mansson R, Zetterblad J, Smith E, Basta B, Bryder D, Akerblad P, Sigvardsson M. The CXCL12, periostin and CCL9 genes are direct targets for early B-cell factor (EBF) in OP-9 stroma cells. J Biol Chem. 2007 Mar 20;.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Rat
Immunogen:
The immunogen for this antibody is: C-HNQLPALANTSV
NCBI Gene ID #:
1879
NCBI Official Name:
early B-cell factor 1
NCBI Official Symbol:
EBF1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_076870.1
Protein GI Number:
31415878
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Signal Transduction
SPECIFICITY:
This antibody is expected not to cross-react with EBF2, EBF3 and EBF4.
Swissprot #:
Q9UH73