ELMO3 Antibody

ProSci
Product Code: PSI-45-532
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-532-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-532 staining (0.1ug/ml) of Human Brain (Hipoocampus) lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.
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45-532 staining (0.1ug/ml) of Human Brain (Hipoocampus) lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.

Further Information

Additional Names:
ELMO3, engulfment and cell motility 3 (ced-12 homolog, C. elegans), CED12, FLJ13824, ced-12 homolog 3, engulfment and cell motility 3, CED-12, ELMO-3
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 75kDa band observed in Human Brain (Hippocampus) lysates (calculated MW of 70.0kDa according to NP_ 078988.2). Recommended concentration: 0.1-0.3ug/ml.Immunohistochemistry:In paraffin embedded Human Prostate shows vesiculate staining in the cytoplasm of secretory cells. Recommended concentration, 5-10ug/ml.
Background References:
  • Gumienny TL, Brugnera E, Tosello-Trampont AC, Kinchen JM, Haney LB, Nishiwaki K, Walk SF, Nemergut ME, Macara IG, Francis R, Schedl T, Qin Y, Van Aelst L, Hengartner MO, Ravichandran KS. CED-12/ELMO, a novel member of the CrkII/Dock180/Rac pathway, is required for phagocytosis and cell migration. Cell 2001 Oct 5;107(1):27-41
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Dog
Immunogen:
The immunogen for this antibody is: TNFNFCYDCSIAEP
NCBI Gene ID #:
79767
NCBI Official Name:
engulfment and cell motility 3
NCBI Official Symbol:
ELMO3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_078988.2
Protein GI Number:
87298935
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
.
Swissprot #:
Q96BJ8