FHL3 Antibody

ProSci
Product Code: PSI-46-934
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-934-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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46-934 (1ug/ml) staining of Human Skeletal Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

46-934 (1ug/ml) staining of Human Skeletal Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Further Information

Additional Names:
FHL3, SLIM2, four and a half LIM domains 3, LIM-only protein FHL3, MGC19547, MGC23614, MGC8696
Application Note:
Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 30kDa band observed in Human, Mouse and Rat Skeletal Muscle lysates (calculated MW of 31.2kDa according to NP_004459.2). Recommended concentration: 1-3ug/ml.
Background References:
  • Samson T, Smyth N, Janetzky S, Wendler O, Muller JM, Schule R, von der Mark H, von der Mark K, Wixler V. The LIM-only proteins FHL2 and FHL3 interact with alpha- and beta-subunits of the muscle alpha7beta1 integrin receptor. J Biol Chem. 2004 Jul 2;279(27):28641-52. Epub 2004 Apr 26.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Pig, Cow
Immunogen:
The immunogen for this antibody is: DQVLCQGCSQAGP
NCBI Gene ID #:
2275
NCBI Official Name:
four and a half LIM domains 3
NCBI Official Symbol:
FHL3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_004459.2
Protein GI Number:
54112385
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
.
Swissprot #:
Q13643