PAR6 alpha Antibody

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ProSci
Product Code: PSI-46-130
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-130-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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46-130 (10ug/ml) staining of paraffin embedded Human Pancreas. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
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46-130 Flow cytometric analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) f
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46-130 (10ug/ml) staining of paraffin embedded Human Pancreas. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
46-130 Flow cytometric analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) f

Documents

Further Information

Additional Names:
PARD6A, PAR6alpha, PAR-6, TAX40, PAR-6A, TIP-40, par-6 partitioning defective 6 homolog alpha (C.elegans), Tax interaction protein 40, par-6 (partitioning defective 6, C.elegans) homolog alpha, PAR6, PAR6C, Tax-interacting protein 40, par-6 partitioning defective 6 homolog alpha, partitioning defective-6 homolog alpha, partitioning-defective protein 6
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 40kDa band observed in lysates of cell lines Jurkat and glioblastoma U251 (calculated MW of 37.4kDa according to NP_058644.1) . Recommended concentration: 1-3ug/ml. Primary incubation 1 hour at room temperature.Immunohistochemistry:In paraffin embedded Human Pancreas, shows strong cytoplasmic staining in rare cells of intralobular ducts. Recommended concentration: 10ug/ml. Also tested in paraffin embedded Human Heart where it shows speckled staining on myocardial fibres in transverse section. Recommended concentration: 5-10ug/ml.Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10ug/ml.
Background References:
  • Johansson A, Driessens M, Aspenstrom P. The mammalian homologue of the Caenorhabditis elegans polarity protein PAR-6 is a binding partner for the Rho GTPases Cdc42 and Rac1. J Cell Sci. 2000 Sep;113 ( Pt 18):3267-75.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-GSRIRGDGSGFSL
NCBI Gene ID #:
50855
NCBI Official Name:
par-6 family cell polarity regulator alpha
NCBI Official Symbol:
PARD6A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_058644.1, NP_001032358.1
Protein GI Number:
8394417
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
This antibody is expected to recognise both reported isoforms (NP_058644.1; NP_001032358.1).
Swissprot #:
Q9NPB6