Eotaxin-2 Antibody
| Code | Size | Price |
|---|
| PSI-XP-5125-0.1mg | 0.1mg | £524.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Mouse
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Neutralisation
- Western Blot (WB)
Storage:
Eotaxin-2 antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated fre
Documents
Further Information
Additional Names:
CKb-6, MPIF-2, Scya24, C-C motif chemokine 24, Eosinophil chemotactic protein 2,
Application Note:
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of mEotaxin-2 (100 ng/mL), a concentration of 7.0-8.0 μg/mL of this antibody is required.
ELISA:
To detect murine Eotaxin-2 by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant murine Eotaxin-2.
Sandwich: To detect mEotaxin-2 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Murine Eotaxin-2 (XP-5125Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mEotaxin-2.
Western Blot:
To detect murine Eotaxin-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant murine Eotaxin-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To yield one-half maximal inhibition [ND50] of the biological activity of mEotaxin-2 (100 ng/mL), a concentration of 7.0-8.0 μg/mL of this antibody is required.
ELISA:
To detect murine Eotaxin-2 by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant murine Eotaxin-2.
Sandwich: To detect mEotaxin-2 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Murine Eotaxin-2 (XP-5125Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mEotaxin-2.
Western Blot:
To detect murine Eotaxin-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant murine Eotaxin-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Background:
Eotaxin is a 74 amino acid, eosinophil chemotactic CC chemokine originally found in bronchoalveolar lavage fluid from allergic inflammatory subjects. It is involved in regulating the recruitment and activation of inflammatory leukocytes, particularly eosinophils. Eotaxin binds and activates the CCR3 chemokine receptor, and may play a fundamental role in the development of allergic responses.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Produced from sera of goats pre-immunized with highly pure (>98%) recombinant murine Eotaxin-2.
NCBI Gene ID #:
56221
NCBI Official Name:
chemokine (C-C motif) ligand 24
NCBI Official Symbol:
Ccl24
NCBI Organism:
Mus musculus
Physical State:
Lyophilized
Protein Accession #:
Q9JKC0
Protein GI Number:
13431902
Purification:
Anti-murine Eotaxin-2 specific antibody was purified by affinity chromatography employing immobilized murine Eotaxin-2 matrix.
Research Area:
Chemokines & Cytokines,Antibody Pairs, Functional Assays
Swissprot #:
Q9JKC0
User NOte:
Centrifuge vial prior to opening.