Leptin Antibody
| Code | Size | Price |
|---|
| PSI-XP-5213-0.1mg | 0.1mg | £524.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Rat
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
Leptin antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze
Documents
Further Information
Additional Names:
OB, obese, Ob, Leptin, Obesity factor,
Application Note:
ELISA:
To detect rat Leptin by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant rat Leptin.
Sandwich:
To detect Rat Leptin by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Rat Leptin (XP-5213Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat Leptin.
Western Blot:
To detect rat Leptin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant rat Leptin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To detect rat Leptin by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant rat Leptin.
Sandwich:
To detect Rat Leptin by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Rat Leptin (XP-5213Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat Leptin.
Western Blot:
To detect rat Leptin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant rat Leptin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Background:
Leptin plays a critical role in the regulation of body weight by inhibiting food intake and stimulating energy expenditure. Defects in Leptin production cause severe hereditary obesity in rodents and humans. In addition to its effects on body weight, leptin has a variety of other functions, including the regulation of hematopoiesis, angiogenesis, wound healing, and the immune and inflammatory response. The Leptin gene is the human homolog of the gene (ob) mutant in the mouse 'obese' phenotype.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Produced from sera of goats pre-immunized with highly pure (>98%) recombinant rat Leptin (rat Leptin).
NCBI Gene ID #:
25608
NCBI Official Name:
leptin
NCBI Official Symbol:
Lep
NCBI Organism:
Rattus norvegicustus norvegicus
Physical State:
Lyophilized
Protein Accession #:
P50596
Protein GI Number:
1709436
Purification:
Anti-rat Leptin specific antibody was purified by affinity chromatography employing immobilized rat Leptin matrix.
Research Area:
Signal Transduction,Growth Factor,Obesity,Chemokines & Cytokines,Antibody Pairs
Swissprot #:
P50596
User NOte:
Centrifuge vial prior to opening.