IL-21 Antibody
| Code | Size | Price |
|---|
| PSI-38-134-0.05mg | 0.05mg | £524.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
IL-21 antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-
Documents
Further Information
Additional Names:
Za11, IL-21Interleukin-21, Za11, IL-21
Application Note:
ELISA:
Sandwich:
To detect hIL-21 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our polyclonal Anti-Human IL-21 as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-21.
Western Blot:
To detect hIL-21 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-21 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Sandwich:
To detect hIL-21 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our polyclonal Anti-Human IL-21 as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-21.
Western Blot:
To detect hIL-21 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-21 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Background:
A novel cytokine related to IL2 and IL15 was recently identified and designated IL21. IL21 has been found to be a powerful growth factor for naive B cells. The receptor for IL21 (IL21R, also termed NILR for novel Interleukin receptor) is a new member of the class I cytokine receptor family. IL21R forms a complex with the common cytokine receptor g chain, gc, and mediates IL21 signaling. Both IL21R and the gc are necessary for the IL21 function. IL21 and its receptor activate JAKSTAT signaling pathway. IL21R is expressed in spleen, thymus, natural killer (NK), T and B cell lines. IL21 plays a role in the proliferation and maturation of NK, B and T cell populations.
Concentration:
batch dependent
Conjugate:
Biotin
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIL-21. Human IL-21 specific antibody was purified by affinity chromatography and then biotinylated.
NCBI Gene ID #:
59067
NCBI Official Name:
interleukin 21
NCBI Official Symbol:
IL21
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
Q9HBE4
Protein GI Number:
55976599
Research Area:
Immunology,Chemokines & Cytokines,Antibody Pairs
Swissprot #:
Q9HBE4
User NOte:
Centrifuge vial prior to opening.