TUSC3 Antibody

ProSci
Product Code: PSI-46-721
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-721-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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46-721 (1ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

46-721 (1ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Further Information

Additional Names:
TUSC3, tumor suppressor candidate 3, D8S1992, MGC13453, N33, OST3A, Putative prostate cancer tumor suppressor
Application Note:
Peptide ELISA: antibody detection limit dilution 1:1000.Western Blot:Approx 37kDa band observed in Human Liver lysates (calculated MW of 39.7kDa according to NP_006756.2 and of 39.6kDa according to NP_839952.1). Recommended concentration: 1-3ug/ml. An additional band of unknown identity was also consistently observed at 22kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Background References:
  • Pils D, Horak P, Gleiss A, Sax C, Fabjani G, Moebus VJ, Zielinski C, Reinthaller A, Zeillinger R, Krainer M. Five genes from chromosomal band 8p22 are significantly down-regulated in ovarian carcinoma: N33 and EFA6R have a potential impact on overall survival. Cancer. 2005 Dec 1;104(11):2417-29.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Cow
Immunogen:
The immunogen for this antibody is: PQRQCSVCRQAN
NCBI Gene ID #:
7991
NCBI Official Name:
tumor suppressor candidate 3
NCBI Official Symbol:
TUSC3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_006756.2, NP_839952.1
Protein GI Number:
30410788
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
This antibody is expected to recognise both reported isoforms (NP_006756.2; NP_839952.1).
Swissprot #:
Q13454