ZEB1 Antibody

ProSci
Product Code: PSI-46-932
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-932-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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46-932 (0.05ug/ml) staining of Mouse Heart lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

46-932 (0.05ug/ml) staining of Mouse Heart lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Further Information

Additional Names:
ZEB1, AREB6, zinc finger E-box binding homeobox 1, BZP, DELTA-EF1, MGC133261, NIL-2-A, NIL-2A, NIL2A, TCF8, ZEB, ZFHEP, ZFHX1A, OTTHUMP00000019405, transcription factor 8 (represses interleukin 2 expression), zinc finger homeodomain enhancer-binding protein
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx.100kDa band observed in Mouse Heart lysates (calculated MW of 122kDa according to Human NP_110378.3 and Mouse NP_035676.1). Recommended concentration: 0.05-0.2ug/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Human and Rat Heart for this particular batch.
Background References:
  • Park SM, Gaur AB, Lengyel E, Peter ME. The miR-200 family determines the epithelial phenotype of cancer cells by targeting the E-cadherin repressors ZEB1 and ZEB2. Genes Dev. 2008 Apr 22 (7): 894-907.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Human, Rat
Immunogen:
The immunogen for this antibody is: C-RPQIRQKIENKPLQE
NCBI Gene ID #:
6935
NCBI Official Name:
zinc finger E-box binding homeobox 1
NCBI Official Symbol:
ZEB1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_001121600.1, NP_110378.3
Protein GI Number:
189409130
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Signal Transduction ,Cancer
SPECIFICITY:
This antibody is expected to recognize both reported isoforms (NP_001121600.1; NP_110378.3).
Swissprot #:
P37275