Dynactin Antibody

Product Code: PSI-45-508

Supplier: ProSci

Code	Size	Price

PSI-45-508-0.1mg

0.1mg

£626.00

Quantity:

Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)

Antibody Clonality: Polyclonal

Regulatory Status: RUO

Target Species: Human

Applications:

Enzyme-Linked Immunosorbent Assay (ELISA)
Immunohistochemistry (IHC)
Western Blot (WB)

Storage:

Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

1 / 5

45-508 (1ug/ml) staining of Human Cerebellum lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

2 / 5

3 / 5

4 / 5

45-508 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (b

5 / 5

45-508 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) fol

❮❯

Documents

Further Information

Additional Names:

DCTN1, dynactin 1 (p150, glued homolog, Drosophila), P135, DP-150, DAP-150, p150-glued, p150, Glued (Drosophila) homolog, 150 kDa dynein-associated polypeptide, dynactin 1 (p150, Glued (Drosophila) homolog), HMN7B, OTTHUMP00000202491, dynactin 1, p150, Glued homolog

Application Note:

Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 150kDa band observed in Human Cerebellum lysates (calculated MW of 142kDa according to NP_004073.2). Additional bands between approx. 40-70kDa were consistently observed, and all were successfully blocked by incubation with the immunizing peptide. Recommended concentration 1-2ug/ml. Primary incubation 1 hour at room temperature. Preliminary testing was unsuccessful on Rat Brain and Testis for this particular batch.Immunohistochemistry:In paraffin embedded Human Cerebellum shows pixulate staining of cytoplasm in neuronal cells. Recommended concentration: 2-4ug/ml. A customer found positive results in IHC on Human HeLa cells.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa and U2OS cells. Recommended concentration: 10ug/ml. Immunocytochemsitry: A customer found positive results in ICC on Mouse embryonic fibroblasts (MEF cells).

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Background References:

Holzbaur EL, Tokito MK. Localization of the DCTN1 gene encoding p150Glued to human chromosome 2p13 by fluorescence in situ hybridization. Genomics. 1996 Feb 1;31(3):398-9. No abstract available.

Buffer:

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.

Concentration:

500 ug/mL

Conjugate:

Unconjugated

DISCLAIMER:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Immunogen:

The immunogen for this antibody is: C-QEQLHQLHSRLIS

NCBI Gene ID #:

1639

NCBI Official Name:

dynactin 1

NCBI Official Symbol:

DCTN1

NCBI Organism:

Homo sapiens

Physical State:

Liquid

Protein Accession #:

NP_004073.2, NP_075408.1, NP_001128512.1, NP_001128513.1, NP_001177765.1, NP_001177766.1

Protein GI Number:

13259510

Purification:

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Research Area:

Cancer

SPECIFICITY:

This antibody is expected to recognise all reported isoforms.

Swissprot #:

Q14203