ERG Antibody

ProSci
Product Code: PSI-45-549
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-549-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-549 (0.1ug/ml) staining of MOLT4 lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
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45-549 (0.1ug/ml) staining of MOLT4 lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Further Information

Additional Names:
ERG, v-ets erythroblastosis virus E26 oncogene like (avian), HGNC:3446, erg-3, p55, transcriptional regulator ERG (transforming protein ERG), v-ets avian erythroblastosis virus E26 oncogene related, v-ets erythroblastosis virus E26 oncogene like, v-ets erythroblastosis virus E26 oncogene homolog (avian), OTTHUMP00000115970, OTTHUMP00000208036, TMPRSS2-ERG prostate cancer specific, ets-related
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 55kDa band observed in lysates of cell line MOLT4 (calculated MW of 53.8kDa according to NP_891548.1). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.
Background References:
  • Tomlins SA, Rhodes DR, Perner S, Dhanasekaran SM, Mehra R, Sun XW, Varambally S, Cao X, Tchinda J, Kuefer R, Lee C, Montie JE, Shah RB, Pienta KJ, Rubin MA, Chinnaiyan AM. Recurrent fusion of TMPRSS2 and ETS transcription factor genes in prostate cancer. Science. 2005 Oct 28;310(5748):644-8.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat
Immunogen:
The immunogen for this antibody is: C-PNTRLPTSHMPSH
NCBI Gene ID #:
2078
NCBI Official Name:
v-ets avian erythroblastosis virus E26 oncogene homolog
NCBI Official Symbol:
ERG
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_891548.1, NP_004440.1, NP_001129626.1, NP_001129627.1, NP_001230358.1|
Protein GI Number:
33667107
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
This antibody is expected to recognise all reported isoforms (NP_891548.1; NP_004440.1; NP_001129626.1; NP_001129627.1; NP_001230358.1)
Swissprot #:
P11308