GADD34 Antibody
Code | Size | Price |
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PSI-45-649-0.1mg | 0.1mg | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Mouse
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.
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Further Information
Additional Names:
PPP1R15A, GADD34, protein phosphatase 1, regulatory (inhibitor) subunit 15A, growth arrest and DNA-damage-inducible 34, protein phosphatase 1, regulatory subunit 15A
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 75kDa band observed in lysates of cell line HepG2 (calculated MW of 73.4kDa according to NP_055145.2). Recommended concentration: 0.3-1ug/ml. Primary incubation 1 hour at room temperature. Negative Control: KLY (Kelly Human neuroblastoma) cell lysate.Immunohistochemistry:In paraffin embedded Human Liver shows cytoplasmic staining consistent with ER in the hepatocytes. Recommended concentration: 2-4ug/ml.Immunofluorescence: Strong expression of the protein seen in the nuclei of HeLa and HepG2 cells. Recommended concentration: 10ug/ml.
Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 10ug/ml.
Background References:
- Kojima E, Takeuchi A, Haneda M, Yagi A, Hasegawa T, Yamaki K, Takeda K, Akira S, Shimokata K, Isobe K. The function of GADD34 is a recovery from a shutoff of protein synthesis induced by ER stress: elucidation by GADD34-deficient mice. FASEB J. 2003 Aug;17(11):1573-5.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-AAALDLSGRRG
NCBI Gene ID #:
23645
NCBI Official Name:
protein phosphatase 1, regulatory subunit 15A
NCBI Official Symbol:
PPP1R15A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_055145.3
Protein GI Number:
9790903
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
.
Swissprot #:
O75807