EGF Antibody

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ProSci
Product Code: PSI-XP-5120Bt
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-XP-5120Bt-0.05mg0.05mg£524.00
Quantity:
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Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Neutralisation
  • Western Blot (WB)
Storage:
EGF antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-th

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To detect mEGF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 ? 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci?s Polyclonal Anti-Murine EGF (XP-5120) as a capture antibody, allows the detection of at least 0.2 ? 0.4 ng/well of recombinant mEGF.
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To detect mEGF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 ? 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci?s Polyclonal Anti-Murine EGF (XP-5120) as a capture antibody, allows the detection of at least 0.2 ? 0.4 ng/well of recombinant mEGF.

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Further Information

Additional Names:
AI790464Pro-epidermal growth factorEGF
Application Note:
Neutralization:

To yield one-half maximal inhibition [ND50] of the biological activity of Murine EGF (0.1 ng/mL), a concentration of 3.0-5.0 ng/mL of this antibody is required.ELISA:

Western Blot:
To detect mEGF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mEGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Concentration:
batch dependent
Conjugate:
Biotin
Immunogen:
Produced from sera of goats pre-immunized with highly pure (>98%) recombinant mEGF (murine EGF).
NCBI Gene ID #:
13645
NCBI Official Name:
epidermal growth factor
NCBI Official Symbol:
Egf
NCBI Organism:
Mus musculus
Physical State:
Lyophilized
Protein Accession #:
P01132
Protein GI Number:
341940478
Purification:
Anti-mEGF specific antibody was purified by affinity chromatography and then biotinylated.
Research Area:
Growth Factor,Antibody Pairs, Functional Assays
Swissprot #:
P01132
User NOte:
Centrifuge vial prior to opening.