RELMa Antibody

ProSci
Product Code: PSI-38-148
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-38-148-0.05mg0.05mg£524.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
RELMa antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-

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To detect Murine RELM-alpha by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 ? 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci?s Polyclonal Anti-Murine RELM-alpha (38-148) as a capture antibody, allows the detection of at least 0.2 ? 0.4 ng/well of recombinant Murine RELM-alpha.

To detect Murine RELM-alpha by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 ? 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci?s Polyclonal Anti-Murine RELM-alpha (38-148) as a capture antibody, allows the detection of at least 0.2 ? 0.4 ng/well of recombinant Murine RELM-alpha.

Further Information

Additional Names:
HIMF, Xcp2, Fizz1, RELMa, Fizz-1, RELMalpha, RELM-alpha, 1810019L16Rik, Himf, Pmng1, Resistin-like alpha, Cysteine-rich secreted protein A12-gamma,
Application Note:
ELISA:

Sandwich:
To detect mRELMa by sandwich ELISA (using 100 μ/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our polyclonal Anti-Murine RELMa as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mRELMa.

Western Blot:
To detect mRELMa by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mRELMa is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Background:
A family of resistin-like molecules (RELMs) has been identified in rodents and humans. RELM alpha belongs to a unique family of tissue-specific cytokines termed FIZZ (found in inflammatory zone) and RELM. The three known members of this family; Resistin, RELM alpha and RELM beta are 85-94 amino acid secreted proteins sharing a conserved C-terminal domain. RELM alpha and Resistin are secreted exclusively by adipocytes while RELM beta is expressed in the epithelium of the colon and small bowel. The RELMs together with resistin comprise a class of tissue-specific signaling molecules. The physiological role and molecular targets of RELM alpha are still unknown.
Concentration:
batch dependent
Conjugate:
Biotin
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mRELMα. Murine RELMα specific antibody was purified by affinity chromatography and then biotinylated.
NCBI Gene ID #:
57262
NCBI Official Name:
resistin like alpha
NCBI Official Symbol:
Retnla
NCBI Organism:
Mus musculus
Physical State:
Lyophilized
Protein Accession #:
Q9EP95
Protein GI Number:
17380316
Research Area:
Signal Transduction,Growth Factor,Antibody Pairs
Swissprot #:
Q9EP95
User NOte:
Centrifuge vial prior to opening.