SNX1 Antibody

ProSci
Product Code: PSI-42-279
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-42-279-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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42-279 (1ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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42-279 (1ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Further Information

Additional Names:
SNX1, sorting nexin 1, HsT17379, MGC8664, SNX1A, Vps5, sorting nexin 1A
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 75kDa band observed in Human lysates of cell line HeLa (calculated MW of 59.1kDa according to NP_003090.2). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 1-3ug/ml.Immunohistochemistry:In paraffin embedded Human Uterus shows vesicular staining in the cytoplasm of endometrial epithelium. Recommended concentration, 3-5ug/ml.Immunocytochemistry: Vesicular staining of SNX1 (green) in formaldehyde-fixed SH5Y5Y. Recommended concentration, 3-5ug/ml.
Background References:
  • Mari M, Bujny MV, Zeuschner D, Geerts WJ, Griffith J, Petersen CM, Cullen PJ, Klumperman J, Geuze HJ, SNX1 defines an early endosomal recycling exit for sortilin and mannose 6-phosphate receptors. Traffic (Copenhagen, Denmark) 2008 Mar 9 (3): 380-93.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-TNSSKPQPTYEELE
NCBI Gene ID #:
6642
NCBI Official Name:
sorting nexin 1
NCBI Official Symbol:
SNX1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_003090.2, NP_690039.1
Protein GI Number:
23111034
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Signal Transduction
SPECIFICITY:
This antibody is expected to recognize isoform a and c (NP_003090.2; NP_690039.1.
Swissprot #:
Q13596