CaM Kinase II Antibody
| Code | Size | Price |
|---|
| PSI-XPS-1000-0.1mL | 0.1mL | £686.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Amphibian
- Human
- Mouse
- Rat
Application: Western Blot (WB)
Storage:
For long term storage ?80˚C is recommended, but shorter term storage at ?20˚C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year.
Documents
Further Information
Application Note:
Antibody dilutions and tissue load should be based on tissue type and expected phosphorylation state. Western blots of rat brain lysates with phospho- and dephosphopeptide competition were used to establish the phospho-specificity of the antibody. Applications include Dot Blot (DB) and Western Blot (WB). Dot Blots and Western Blots performed with each lot and working dilutions internally tested under ideal conditions for DB and WB were 1:1000. Suitability for Immunohistochemistry (IHC) has not been determined. Human, mouse, rat and Xenopus have 100% amino acid sequence identity with the antigen used to raise the antibody.
Background:
Calcium/calmodulin kinase II (CaM Kinase II) is multi-functional calcium and calmodulin-dependent protein kinase that mediates cellular responses to second messengers. Phosphorylation of Thr286 produces an autonomously active form of CaM Kinase II. The antibody was raised against a synthetic phosphopeptide corresponding to amino acid residues surrounding the phosphorylated-Thr286 found in rat brain CaM Kinase II.
Background References:
- Kennedy, M. B., "Signal-processing machines at the postsynaptic density," Science, 290 (2000) 750-754.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
CaM Kinase II (Thr286) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acid residues surrounding the phosphorylated-Thr286 found in rat brain CaM Kinase II.
Modifications:
Phospho-Specific
NCBI Gene ID #:
25400
NCBI Official Name:
calcium/calmodulin-dependent protein kinase II alpha
NCBI Official Symbol:
Camk2a
NCBI Organism:
Rattus norvegicus
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
50/60
Protein Accession #:
P11275
Protein GI Number:
125285
Purification:
Affinity Purified
Research Area:
Signal Transduction ,Phospho-Specific
SPECIFICITY:
CaM Kinase II antibody is specific for a 50 kDa and b 60 kDa CaM Kinase II phosphorylated at Thr286.
Swissprot #:
P11275
User NOte:
Optimal dilutions for each application to be determined by the researcher.