Cdc2 Antibody

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ProSci
Product Code: PSI-XPS-1003
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-XPS-1003-0.1mL0.1mL£686.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Amphibian
  • Canine (dog)
  • Human
  • Mouse
  • Rat
Application: Western Blot (WB)
Storage:
For long term storage ?80˚C is recommended, but shorter term storage at ?20˚C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year.

Images

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Western blot of human T47D cells showing phospho-specific immunolabeling of the ~34k cdc2 protein phosphorylated at Tyr15. In lane 2, cells were treated with EGF leading to dephosphorylation of Tyr15.
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Western blot of human T47D cells showing phospho-specific immunolabeling of the ~34k cdc2 protein phosphorylated at Tyr15. In lane 2, cells were treated with EGF leading to dephosphorylation of Tyr15.

Documents

Further Information

Application Note:
The antibody is purified by sequential chromatography on phospho- and non-phosphopeptide affinity columns. Antibody dilutions and tissue load should be based on tissue type and expected phosphorylation state. Western blots of SK-N-MC total cell extracts with phospho- and non-phosphopeptide competition were used to establish the phospho-specificity of the antibody. Immunolabeling of the Mr 34 kDa cdc2 protein is blocked by the Ser15 phosphopeptide used as antigen but not by the corresponding non-phosphopeptide. Initial recommended range of dilutions: 1:500 to 1:2000. Applications include Dot Blots (DB) and Western Blot (WB). Suitability for Immunohistochemistry (IHC) has not been determined. Drosophila, human, mouse, rat and Xenopus have 100% amino acid sequence identity with the antigen used to raise the antibody. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.
Background:
Cdc2 is a highly conserved protein serine kinase that plays a key role in regulation of the cell cycle. The ability of cdc2 to exercise control over the cell cycle is dependent upon the phosphorylation of Tyr15 in cdc2.
Background References:
  • Sible, J.C., "Cell biology: thanks for the memory," Nature (London), 426 (2003) 392-393.
  • Sagata, N., "Molecular biology: untangling checkpoints," Science, 298 (2002) 1905-1907.
  • Nakamizo, A., Inamura, T., Inoha, S., Amano, T., Ochi, H., Ikezaki, K., and Fukui, M., "Suppression of cdc2 dephosphorylation at the tyrosine 15 residue during nitrosourea-induced G2M phase arrest in glioblastoma cell lines," J. Neurooncol., 59 (2002) 7-13.
  • Maller, J.L., "Mitotic control," Curr. Opinion. Cell Biology, 3 (1991) 269-275.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Cdc2 (Tyr15) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acid residues surrounding the phosphorylated-Tyr15 of human, mouse, rat and xenopus cdc2.
Modifications:
Phospho-Specific
NCBI Gene ID #:
365842
NCBI Official Name:
CDC-like kinase 2
NCBI Official Symbol:
Clk2
NCBI Organism:
Rattus norvegicus
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
34
Protein Accession #:
Q5XI98
Protein GI Number:
81910362
Purification:
Affinity Purified
Research Area:
Signal Transduction ,Phospho-Specific
SPECIFICITY:
Cdc2 antibody is specific for cdc2 phosphorylated at Tyr15.
Swissprot #:
Q5XI98
User NOte:
Optimal dilutions for each application to be determined by the researcher.