GATA1 Antibody

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ProSci
Product Code: PSI-79-120
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-79-120-0.1mg0.1mg£488.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store antibody at -20˚C for up to one year.

Images

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Western blot analysis of lysed extracts from HL60 cells untreated or treated with PMA using GATA1 (Phospho-Ser142).
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<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using GATA1 (Phospho-Ser142).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells using GATA1 (Phospho-Ser142).
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Western blot analysis of lysed extracts from HL60 cells untreated or treated with PMA using GATA1 (Phospho-Ser142).
<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using GATA1 (Phospho-Ser142).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells using GATA1 (Phospho-Ser142).

Documents

Further Information

Additional Names:
GF1, GF-1, NFE1, XLTT, ERYF1, NF-E1, XLANP, XLTDA, GATA-1, CREB2, TXREB, GAT1, GATA1
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Background:
GATA1 encodes a protein which belongs to the GATA family of transcription factors. The protein plays an important role in erythroid development by regulating the switch of fetal hemoglobin to adult hemoglobin. Mutations in this gene have been associated with X-linked dyserythropoietic anemia and thrombocytopenia.
Background References:
  • Zon LI, et al. (1990) Proc Natl Acad. Sci USA. 87: 668-672. Trainor C D, et al. (1990) Nature. 343: 92-96. Nichols K E, et al. (2000) Nat Genet. 24: 266-270.
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
GATA1 (Phospho-Ser142) antibody was raised against a peptide sequence around phosphorylation site of serine 142 (R-L-S (p) -P-D) derived from Human GATA1.
Modifications:
Phospho-Specific
NCBI Gene ID #:
2623
NCBI Official Name:
GATA binding protein 1 (globin transcription factor 1)
NCBI Official Symbol:
GATA1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
43 kDa
Protein Accession #:
NP_002040.1
Protein GI Number:
4503925
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Research Area:
Phospho-Specific
SPECIFICITY:
This antibody detects endogenous level of GATA1 only when phosphorylated at serine 142.
Swissprot #:
P15976