GSK3a Antibody

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ProSci
Product Code: PSI-79-105
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-79-105-0.1mg0.1mg£488.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store antibody at -20˚C for up to one year.

Images

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Western blot analysis of lysed extracts from 293 cells untreated (Lane 1) or treated with serum (lane 2) using GSK3α (Phospho-Ser21).
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<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using GSK3α (Phospho-Ser21).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic staining using GSK3α (Phospho-Ser21).
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Western blot analysis of lysed extracts from 293 cells untreated (Lane 1) or treated with serum (lane 2) using GSK3α (Phospho-Ser21).
<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using GSK3α (Phospho-Ser21).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic staining using GSK3α (Phospho-Ser21).

Documents

Further Information

Additional Names:
MAPTL, MTBT1, TAU, Factor A, GSK-3 alpha, kinase GSK3-alpha
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Background:
Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN.
Background References:
  • Barry FA, et al. (2003) FEBS Lett. 553 (1-2): 173-178. Koivisto L, et al. (2003) J Cell Sci. 116 (Pt 18): 3749-3760. Welsh G I, et al. (1996) Trends Cell Biol. 6:274-279. Srivastava A K, et al. (1998) Mol. Cell. Biochem. 182: 135-141.
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
GSK3a (Phospho-Ser21) antibody was raised against a peptide sequence around phosphorylation site of serine 21 (T-S-S (p) -F-A) derived from Human GSK3α.
Modifications:
Phospho-Specific
NCBI Gene ID #:
2931
NCBI Official Name:
glycogen synthase kinase 3 alpha
NCBI Official Symbol:
GSK3A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
51 kDa
Protein Accession #:
NP_063937.2
Protein GI Number:
49574532
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Research Area:
Phospho-Specific,Apoptosis,Cancer,Cell Cycle
SPECIFICITY:
This antibody detects endogenous level of GSK3α only when phosphorylated at serine 21and does not detect GSK-3β when phosphorylated at Ser9.
Swissprot #:
P49840