Interferon-a Receptor

Contact us
ProSci
Product Code: PSI-XPS-1014
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-XPS-1014-0.1mL0.1mL£686.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Bovine (Cattle)
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
For long term storage ?80˚C is recommended, but shorter term storage at ?20˚C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year.

Images

1 / 1
Western blot of immunoprecipitates from HEK 293 cells transfected with: 1. Mock 2. IFNAR1 WT and 3. IFNAR1 Ser535Arg and Ser 539Art mutants. The immunolabeling is absent in the IFNAR1 Ser535 and Ser539 mutants. The labeling is blocked by the phosphopeptide (Phos) used as the antigen but not by the dephosphopeptide (Dephos).
❮❯

Western blot of immunoprecipitates from HEK 293 cells transfected with: 1. Mock 2. IFNAR1 WT and 3. IFNAR1 Ser535Arg and Ser 539Art mutants. The immunolabeling is absent in the IFNAR1 Ser535 and Ser539 mutants. The labeling is blocked by the phosphopeptide (Phos) used as the antigen but not by the dephosphopeptide (Dephos).

Documents

Further Information

Application Note:
Applications include Dot Blots (DB) and Western Blots (WB). Suitability for Immunohistochemistry (IHC) has not yet been determined. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.
Background:
Interferons are widely used therapeutic agents because of their anti tumor and antiviral effects and because of their modulatory effects on the immune system. These cytokines produce their effects by binding to the Type 1 Interferon-alpha Receptor (IFNAR1). Down regulation of this receptor plays a key role in determining the magnitude and duration of cytokine signaling. This down regulation is thought to be influenced by phosphorylation of Serine 535 and 539 in the IFNAR1.
Background References:
  • Suresh Kumar, K.G., Tang, W., Ravindranath, A.K., Clark, W.A., Croze, E., Fuchs, S.Y., "SCFHOS ubiquitin ligase mediates the ligand-induced down-regulation of the interferon- receptor," The EMBO Journal, 22 (2003), 5480-5490.
  • Kirkwood, J., "Cancer immunotherapy: the interferon- experience," Semin. Oncol., 29 (2002) 18-26.
  • Biron, C.A., "Interferons alpha and beta as immune regulators-a new look," Immunity, 14 (2001) 661-664.
  • Stark, G.R., Kerr, I.M., Williams, B.R., Silverman, R.H. and Schreiber, R.D. "How cells respond to interferons." Annu. Rev. Biochem., 67 (1998) 227-264.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
IFNAR1 (Ser535/539) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acid residues surrounding the human Phospho-Ser535, 539 Type I Interferon-a Receptor Subunit 1 (IFNAR1).
Modifications:
Phospho-Specific
NCBI Gene ID #:
3454
NCBI Official Name:
interferon (alpha, beta and omega) receptor 1
NCBI Official Symbol:
IFNAR1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
110-130
Protein Accession #:
P17181
Protein GI Number:
90110827
Purification:
Affinity Purified
Research Area:
Signal Transduction ,Phospho-Specific
SPECIFICITY:
Interferon a Receptor antibody is specific for IFNAR1 phosphorylated at Ser535,539.
Swissprot #:
P17181
User NOte:
Optimal dilutions for each application to be determined by the researcher.