MEK1/2 Antibody

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ProSci
Product Code: PSI-XPS-1018
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-XPS-1018-0.1mL0.1mL£686.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Amphibian
  • Human
  • Mouse
  • Rat
Application: Western Blot (WB)
Storage:
For long term storage ?80˚C is recommended, but shorter term storage at ?20˚C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year.

Images

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Western blot of NIH 3T3 cell lysates. The cells were serum starved (control) or incubated in the presence of serum (serum) for 5 minutes. The western blot shows the immunolabeling of the ~45k MEK1/2 was absent in control and stimulated by serum.
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Western blot of NIH 3T3 cell lysates. The cells were serum starved (control) or incubated in the presence of serum (serum) for 5 minutes. The western blot shows the immunolabeling of the ~45k MEK1/2 was absent in control and stimulated by serum.

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Further Information

Application Note:
The antibody immunolabels the ~45k MEK protein in a Western blot of serum-stimulated NIH 3T3 cells. The immunolabeling is blocked by the phosphopeptide used as antigen but not the corresponding non-phosphopeptide. Applications include Dot Blots (DB) and Western Blots (WB). Suitability for Immunohistochemistry (IHC) has not yet been determined. Human, mouse, rat and Xenopus have 100% amino acid sequence identity with the antigen used to raise the antibody. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.
Background:
MEK 1 and MEK 2 are integral components of the MAP kinase cascade that regulates cell growth and differentiation and this pathway also plays a key role in synaptic plasticity in brain. Activation of MEK 1/2 occurs via phosphorylation of two serine residues (Ser218 and Ser222). Activated MEK 1/2 then acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on ERK. This phosphorylation of ERK by MEK 1/2 is a critical step in the MAP kinase cascade.
Background References:
  • N.G. Ahn, N.G., Robbins, D.G., Haycock, J.W., Seger, R., Cobb, M.H. and Krebs, E.G., "Identification of an activator of the microtubule-associated protein 2 kinases ERK1 and ERK2 in PC12 cells stimulated with nerve growth factor or bradykinin," J. Neurochem. 59 (1992) 147 - 156.
  • Crews, C.M., Alessandrini, A. and Erikson, R.L., "The primary structure of MEK, a protein kinase that phosphorylates the ERK gene product," Science 258 (1992) 478 - 480.
  • Ahn, N.G. "The MAP kinase cascade. Discovery of a new signal transduction pathway," Mol. Cell Biochem. 127 - 128 (1993) 201 - 209.
  • Adams, J.P. and Sweatt, J.D., "Molecular psychology: Roles for the ERK MAP kinase cascade in memory," Annu. Rev. Pharmacol. Toxicol. 42 (2002) 135 - 163.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
MEK 1/2 (Ser218/222) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phosphoSer218/222 of human MEK 1/2.
Modifications:
Phospho-Specific
NCBI Gene ID #:
5604
NCBI Official Name:
mitogen-activated protein kinase kinase 1
NCBI Official Symbol:
MAP2K1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
45
Protein Accession #:
Q02750
Protein GI Number:
400274
Purification:
Affinity Purified
Research Area:
Signal Transduction ,Phospho-Specific
SPECIFICITY:
MEK1/2 antibody is specific for MEK 1/2 phosphorylated at Ser218/222.
Swissprot #:
Q02750
User NOte:
Optimal dilutions for each application to be determined by the researcher.