PLKK Antibody
| Code | Size | Price |
|---|
| PSI-XPS-1022-0.1mL | 0.1mL | £686.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Amphibian
Application: Western Blot (WB)
Storage:
For long term storage ?80˚C is recommended, but shorter term storage at ?20˚C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year.
Documents
Further Information
Application Note:
Applications include Dot Blots (DB) and Western Blots (WB). Suitability for Immunohistochemistry (IHC) has not yet been determined. Xenopus is the only species tested to date. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.
Background:
Considerable evidence indicates that a Polo-Like Kinase (PLK) plays an important role in cell cycle regulation. PLK is also required for bipolar spindle formation, activation of the anaphase-promoting complex/cyclosome, and cytokinesis. Recent work led to the identification of a PLKK that is thought to be responsible for activation of PLK. Recent work (Erikson, et al., 2004) has shown that PLKK is in turn activated by phosphorylation at three sites (Ser482, Ser486 and Ser490). Thus activation of PLK is thought to involve a kinase cascade involving the phosphorylation of Ser482,486,490 in PLKK.
Background References:
- van Vugt, M.A.T.M., van de Weerdt, B.C.M., Vader, G., Janssen, H., Calafat, J., Klompmaker, R., Wolthuis, R.M.F., and Medema, R.H., "Polo-like Kinase-1 Is Required for Bipolar Spindle Formation but Is Dispensable for Anaphase Promoting Complex/Cdc20 Activation and Initiation of Cytokinesis," J. Biol. Chem., 279 (2004) 36,841-36,854.
- Erikson, E., Haystead, T.A.J., Qian, Y-W., and Maller, J.L., "A Feedback Loop in the Polo-like Kinase Activation Pathway," J. Biol. Chem., 279 (2004) 32,219-32,224.
- Liu, J., Lewellyn, A.L., Chen, L.G., and Maller, J.L., "The Polo Box Is Required for Multiple Functions of Plx1 in Mitosis," J. Biol. Chem., 279 (2004) 21,367 - 21,373.
- Kumagai, A., and Dunphy, W. G., "Purification and Molecular Cloning of Plx1, a Cdc25-Regulatory Kinase from Xenopus Egg Extracts," Science 273 (1996) 1377-1380.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
PLKK (Ser482/486/490) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the Xenopus Phospho-Ser482, 486, 490 PLKK.
Modifications:
Phospho-Specific
NCBI Gene ID #:
380481
NCBI Official Name:
polo-like kinase 1
NCBI Official Symbol:
plk1
NCBI Organism:
Xenopus laevis
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
120
Protein Accession #:
P70032
Protein GI Number:
49036444
Purification:
Affinity Purified
Research Area:
Signal Transduction ,Phospho-Specific
SPECIFICITY:
PLKK antibody is specific for PLKK phosphorylated at Ser482,486,490.
Swissprot #:
P70032
User NOte:
Optimal dilutions for each application to be determined by the researcher.