MARCKS Antibody

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ProSci
Product Code: PSI-XPS-1015
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-XPS-1015-0.1mL0.1mL£686.00
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Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Amphibian
  • Human
  • Mouse
  • Rat
Application: Western Blot (WB)
Storage:
For long term storage ?80˚C is recommended, but shorter term storage at ?20˚C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year.

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Western blot of rat brain lysate showing phospho-specific immunolabeling of the ~87k MARCKS protein.
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Western blot of rat brain lysate showing phospho-specific immunolabeling of the ~87k MARCKS protein.

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Further Information

Application Note:
Immunolabeling of the 87kDa band in rat brain homogenate is blocked by the Ser152/156 phosphopeptide but not by the non-phosphopeptide. The antibody is purified by sequential chromatography on phospho- and non-phosphopeptide affinity columns. Antibody dilutions and tissue load should be based on tissue type and expected phosphorylation state. Initial recommended range of dilutions: 1:500 to 1:2000. Applications include Dot Blots (DB) and Western Blot (WB). Suitability for Immunohistochemistry (IHC) not yet determined. Human, mouse, rat, and Xenopus have 100% amino acid sequence identity with the antigen used to raise the antibody. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.
Background:
Myristoylated Alanine-Rich C Kinase Substrate (MARCKS) is a major substrate for phosphorylation by protein kinase C (PKC). The phosphorylation of Ser152/156 can be used as a measure of PKC activation. Moreover, MARCKS is a member of a family of calmodulin binding proteins. Phosphorylation of Ser152/156 modulates the binding of MARCKS to calmodulin.
Background References:
  • Palmer, R.H., Schönwasser, D.C., Rahman, D., Pappin, D.J.C., Herget, T., and Parker, P.J., "PRK1 phosphorylates MARCKS at the PKC sites: Serine 152, serine 156 and serine 163," FEBS Letters, 378 (1996) 281 - 285.
  • Spizz, G. and Blackshear, P.J., "Protein kinase C-mediated phosphorylation of the myristoylated alanine-rich C-kinase substrate protects it from specific proteolytic cleavage," J. Biol. Chem., 271 (1996) 553 - 562.
  • Thelen, M., Rosen, A., Nairn, A.C., and Aderem, A., "Regulation by phosphorylation of reversible association of a myristoylated protein kinase C substrate with the plasma membrane," Nature (London), 351 (1991) 320 - 322.
  • Hartwig, J.H., Thelen, M., Rosen, A., Janmey, P.A., Nairn, A.C., and Aderem, A., "MARCKS is an actin filament crosslinking protein regulated by protein kinase C and calcium-calmodulin," Nature (London), 356 (1992) 618 - 622.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
MARCKS (Ser152/156) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser152 and phospho-Ser156 of rat MARCKS.
Modifications:
Phospho-Specific
NCBI Gene ID #:
25603
NCBI Official Name:
myristoylated alanine rich protein kinase C substrate
NCBI Official Symbol:
Marcks
NCBI Organism:
Rattus norvegicus
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
87
Protein Accession #:
P30009
Protein GI Number:
266495
Purification:
Affinity Purified
Research Area:
Signal Transduction ,Phospho-Specific ,
SPECIFICITY:
MARCKS antibody is specific for MARCKS phosphorylated at Ser152 and Ser156.
Swissprot #:
P30009
User NOte:
Optimal dilutions for each application to be determined by the researcher.