Vimentin Antibody

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ProSci
Product Code: PSI-50-264
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-50-264-0.1mL0.1mL£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Chicken
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Vimentin antibody can be stored at -20˚C and is stable at -20˚C for at least 1 year.

Images

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Western blot of NIH 3T3 cells showing specific immunolabeling of the ~50k Vimentin protein.<br><br><b>Below: </b>Mixed neuron/glial cultures stained with anti-Vimentin (green) and rabbit anti-GFAP antibody (red). Vimentin is expressed alone in fibroblastic and endothelial cells, which are the flattened cells in the middle of the image which appear green. Astrocytes may express primarily GFAP, or GFAP and vimentin, and so appear red (GFAP only) or golden yellow (GFAP and Vimentin). In cells which express both GFAP and vimentin, the two proteins assemble to produce heteropolymer filaments.
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Western blot of NIH 3T3 cells showing specific immunolabeling of the ~50k Vimentin protein.<br><br><b>Below: </b>Mixed neuron/glial cultures stained with anti-Vimentin (green) and rabbit anti-GFAP antibody (red). Vimentin is expressed alone in fibroblastic and endothelial cells, which are the flattened cells in the middle of the image which appear green. Astrocytes may express primarily GFAP, or GFAP and vimentin, and so appear red (GFAP only) or golden yellow (GFAP and Vimentin). In cells which express both GFAP and vimentin, the two proteins assemble to produce heteropolymer filaments.

Documents

Further Information

Background:
Vimentin is the major protein subunit of the 10nm or intermediate filaments (IFs) found in many kinds of mesenchymal and epithelial cells as well as developing neuronal and astrocytic precursor cells in the CNS. Vimentin is thought to be critically involved in lymphocyte adhesion and transmigration (Nieminen M et al. 2006). Copolymers are frequently formed between vimentin and other IFs, such as GFAP (in many kinds of astrocytes), desmin (in muscle cells) and neurofilament proteins (in developing neurons). Antibodies to vimentin are useful in studies of stem cells and generally to reveal the filamentous cytoskeleton. Recent studies suggest that vimentin affects prostate cancer cells motility and invasiveness (Zhao et al. 2008).
Background References:
  • Nieminen M, Henttinen T, Merinen M, Marttila-Ichihara F, Eriksson JE, Jalkanen S (2006) Vimentin function in lymphocyte adhesion and transcellular migration. Nat Cell Biol 8(2):156-62.
  • Zhao Y, Yan Q, Long X, Chen X, Wang Y (2008) Vimentin affects the mobility and invasiveness of prostate cancer cells. Cell Biochem Funct. May 8
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Recombinant human Vimentin purified from E. coli.
NCBI Gene ID #:
7431
NCBI Official Name:
vimentin
NCBI Official Symbol:
VIM
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
50
Protein Accession #:
P08670
Protein GI Number:
55977767
Purification:
Total IgY fraction
Research Area:
Stem Cell ,Cancer
SPECIFICITY:
Specific for the ~50kDa Vimentin protein.
Swissprot #:
P08670
User NOte:
Optimal dilutions for each application to be determined by the researcher.