ABHD5 Antibody

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ProSci
Product Code: PSI-45-186
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-186-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Bovine (Cattle)
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-186 (0.2ug/ml) staining of NIH3T3 lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
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45-186 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and vesicle/ cytoplasmic staining. The nuc
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45-186 (0.2ug/ml) staining of NIH3T3 lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
45-186 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and vesicle/ cytoplasmic staining. The nuc

Documents

Further Information

Additional Names:
ABHD5, CGI58 protein, aCGI58, bhydrolase domain containing 5, CDS, IECN2, MGC8731, NCIE2
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 40kDa band observed in lysates of cell lines NIH3T3/3TL (calculated MW of 39.1kDa according to NP_057090.2). Additional faint bands of 26kDa and 18kDa were also observed, and were successfully blocked by incubation with the immunizing peptide. Recommended concentration: 0.2-0.6ug/ml Primary incubation 1 hour at room temperature.Immunohistochemistry:Paraffin embedded Human Pancreas and Kidney. Recommended concentration: 3.75ug/ml.Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasm/ vesicles of U2OS cells. Recommended concentration: 10ug/ml.
Background References:
  • Lass A, Zimmermann R, Haemmerle G, Riederer M, Schoiswohl G, Schweiger M, Kienesberger P, Strauss JG, Gorkiewicz G, Zechner R. Adipose triglyceride lipase-mediated lipolysis of cellular fat stores is activated by CGI-58 and defective in Chanarin-Dorfman Syndrome. Cell Metab. 2006 May;3(5):309-19.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Rat, Dog
Immunogen:
The immunogen for this antibody is: C-FPERPDLADQDR
NCBI Gene ID #:
51099
NCBI Official Name:
abhydrolase domain containing 5
NCBI Official Symbol:
ABHD5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_057090.2
Protein GI Number:
31542303
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
This antibody is not expected to cross-react with ABHD4.
Swissprot #:
Q8WTS1