AGTR1 Antibody

ProSci
Product Code: PSI-45-216
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-216-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-216 (0.2ug/ml) staining o fHuman Liver (A) + peptide (B), Mouse Liver (C) + peptide (D) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

45-216 (0.2ug/ml) staining o fHuman Liver (A) + peptide (B), Mouse Liver (C) + peptide (D) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Further Information

Additional Names:
AGTR1, AT1, angiotensin II receptor, type 1 , AG2S, AGTR1A, AGTR1B, AT1B, AT2R1, AT2R1A, AT2R1B, HAT1R, angiotensin receptor 1, angiotensin receptor 1B, type-1B angiotensin II receptor
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 38kDa band observed in Human and Mouse Liver lysates (calculated MW of 41.1kDa according to Human NP_000676.1 and 40.9kDa according to Mouse NP_796296.1) Additional bands of unknown identity were also observed at 45 and 75kDakDa and were successfully blocked by incubation with the immunizing peptide. Recommended concentration: 0.1-0.3ug/ml. Primary incubation 1 hour at room temperature.
Background References:
  • Wang M, Jiang C, Wang C, Yao Q. The Role of brain angiotensinergic AT(1) receptor in the carbachol-induced natriuresis and expression of nNOS in the Locus Coeruleus (LC) and proximal convoluted tubule (PCT). Physiol Res. 2006 Aug 22;
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-EIQKNKPRNDDIFK
NCBI Gene ID #:
185
NCBI Official Name:
angiotensin II receptor, type 1
NCBI Official Symbol:
AGTR1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_000676.1, NP_004826.2, NP_033611.1, NP_114038.1, NP_114438.1
Protein GI Number:
4501997
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer ,Infectious Disease
SPECIFICITY:
All reported variants represent identical protein (NP_000676.1, NP_004826.2, NP_033611.1, NP_114038.1 and NP_114438.1).
Swissprot #:
P30556