AIF1 Antibody

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ProSci
Product Code: PSI-45-219
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-219-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-219 (1ug/ml) staining of Human (A) and (0.5ug/ml) of Mouse (B) and Rat (C) Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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45-219 (5ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. <strong>This data is from a previous batch, not on sale.</strong>
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45-219 (5ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. <strong>This data is from a previous batch, not on sale.</strong>
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45-219 (5ug/ml) staining of paraffin embedded Human Lung. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining. <strong>This data is from a previous batch, not on sale.</strong>
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45-219 Negative Control showing staining of paraffin embedded Human Lung, with no primary antibody. <strong>This data is from a previous batch, not on sale.</strong>
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45-219 (1ug/ml) staining of Human (A) and (0.5ug/ml) of Mouse (B) and Rat (C) Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
45-219 (5ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. <strong>This data is from a previous batch, not on sale.</strong>
45-219 (5ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. <strong>This data is from a previous batch, not on sale.</strong>
45-219 (5ug/ml) staining of paraffin embedded Human Lung. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining. <strong>This data is from a previous batch, not on sale.</strong>
45-219 Negative Control showing staining of paraffin embedded Human Lung, with no primary antibody. <strong>This data is from a previous batch, not on sale.</strong>

Documents

Further Information

Additional Names:
AIF1, IBLY409840A1, allograft inflammatory factor 1, AIF-1, IRT-1, interferon gamma responsive transcript, ionized calcium-binding adapter molecule, Daintain, DADB-70P7.8
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 17kDa band observed in Human Brain (Frontal Cortex) and Rat Brain lysates (calculated MW of 16.7kDa according to Human NP_001614.3 and 16.8kDa according to Rat NP_058892.1). Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour.This antibody can also be purchased as directly conjugated to HRP. Please specify when you order.
Background References:
  • Autieri MV, Agrawal N. IRT-1, a novel interferon-gamma-responsive transcript encoding a growth-suppressing basic leucine zipper protein. J Biol Chem. 1998 Jun 12;273(24):14731-7.
  • Clavaguera F, Bolmont T, Crowther RA, Abramowski D, Frank S, Probst A, Fraser G, Stalder AK, Beibel M, Staufenbiel M, Jucker M, Goedert M, Tolnay M. Transmission and spreading of tauopathy in transgenic mouse brain. Nat Cell Biol. 2009 Jul;11(7):909-13.
  • Kalm M, Lannering B, Bj?rk-Eriksson T, Blomgren K. Irradiation-induced loss of microglia in the young brain. J Neuroimmunol. 2009 Jan 3;206(1-2):70-
  • Hirko AC, Dallasen R, Jomura S, Xu Y. Modulation of inflammatory responses after global ischemia by transplanted umbilical cord matrix stem cells. Stem Cells. 2008 Nov;26(11):2893-901.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Pig
Immunogen:
The immunogen for this antibody is: C-TGPPAKKAISELP
NCBI Gene ID #:
199
NCBI Official Name:
allograft inflammatory factor 1
NCBI Official Symbol:
AIF1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_116573.1, NP_001614.3
Protein GI Number:
14574566
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
This antibody is expected to recognize isoform 1 (NP_116573.1) and isoform 3 (NP_001614.3).
Swissprot #:
P55008