AIM2 Antibody

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ProSci
Product Code: PSI-42-531
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-42-531-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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42-531 (0.75ug/ml) staining of HeLa cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
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42-531 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic and some nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
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42-531 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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42-531 (0.75ug/ml) staining of HeLa cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
42-531 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic and some nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
42-531 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Documents

Further Information

Additional Names:
AIM2, absent in melanoma 2, PYHIN4
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 50+55kDa bands observed in lysates of Human WI-38 fibroblast after treatment with interferon beta (calculated MW of 39.0kDa according to NP_004824.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Choubey et al., J Interferon Cytokine Res. 2010 Jun;30(6):371-80. PMID: 20187776). Recommended concentration: 1-3ug/ml.Immunohistochemistry:In paraffin embedded Human Spleen shows nucleolar staining in some splenocytes. Recommended concentration, 4-6ug/ml.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-GEKLQLTSGVHSTIK
NCBI Gene ID #:
9447
NCBI Official Name:
absent in melanoma 2
NCBI Official Symbol:
AIM2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_004824.1
Protein GI Number:
4757734
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
Swissprot #:
O14862