AKAP95 Antibody

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ProSci
Product Code: PSI-45-233
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-233-0.1mg0.1mg£626.00
Quantity:
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Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-233 staining (1ug/ml) of Human Liver lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
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45-233 staining (1ug/ml) of Human Liver lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Documents

Further Information

Additional Names:
AKAP8, AKAP95, DKFZp586B1222, A kinase (PRKA) anchor protein 8, A kinase anchor protein 95, A-kinase anchor protein 8, A-kinase anchor protein, 95kDa
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Experiments gave a band at approx 100kDa in Human Liver lysate after 1ug/ml antibody staining. This band corresponds to the 95kDa band reported in literature (PMID: 10601332). This protein has a calculated MW of 76.1kDa according to NP_005849. Recommended concentration: 1-3ug/ml.
Background References:
  • Eide T, Coghlan V, Orstavik S, Holsve C, Solberg R, Skalhegg BS, Lamb NJ, Langeberg L, Fernandez A, Scott JD, Jahnsen T, Tasken K. Molecular cloning, chromosomal localization, and cell cycle-dependent subcellular distribution of the A-kinase anchoring protein, AKAP95. Exp Cell Res. 1998 Feb 1;238(2):305-16.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Cow
Immunogen:
The immunogen for this antibody is: DQGYGGYGAWSAG-C
NCBI Gene ID #:
10270
NCBI Official Name:
A kinase (PRKA) anchor protein 8
NCBI Official Symbol:
AKAP8
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_005849.1
Protein GI Number:
5031579
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
.
Swissprot #:
O43823