AKR1B10 Antibody

ProSci
Product Code: PSI-45-234
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-234-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-234 (0.03ug/ml) staining of A549 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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45-234 (0.03ug/ml) staining of A549 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Further Information

Additional Names:
AKR1B10, aldo-keto reductase family 1, member B10 (aldose reductase) , AKR1B11, AKR1B12, ALDRLn, ARL-1, ARL1, HIS, HIS, MGC14103, aldo-keto reductase family 1, member B10, aldo-keto reductase family 1, member B11 (aldose reductase-like), aldose reductase-like 1N: aldose reductase-like peptide, aldose reductase-related protein, small intestine reductase
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 35kDa band observed in lysates of cell lines HEK293, A549 and HepG2 (calculated MW of 36.0kDa according to NP_064695.2). In transfected HEK293 transiently expressing AKR1B10 a band of approx. 40kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.03-0.1ug/ml.
Background References:
  • Tammali R, Ramana KV, Singhal SS, Awasthi S, Srivastava SK. Aldose reductase regulates growth factor-induced cyclooxygenase-2 expression and prostaglandin E2 production in human colon cancer cells. Cancer Res. 2006 Oct 1;66(19):9705-13.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-QSSHLEDYPFDAE
NCBI Gene ID #:
57016
NCBI Official Name:
aldo-keto reductase family 1, member B10 (aldose reductase)
NCBI Official Symbol:
AKR1B10
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_064695.2
Protein GI Number:
20127592
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
.
Swissprot #:
O60218