ALOX15 Antibody

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ProSci
Product Code: PSI-46-769
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-769-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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46-769 (0.2ug/ml) staining of nuclear HeLa lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
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46-769 (0.2ug/ml) staining of nuclear HeLa lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Documents

Further Information

Additional Names:
ALOX15, arachidonate 15-lipoxygenase, 15-LOX-1, 15-LOX2
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx. 80kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 74.8kDa according to NP_001131.3). Recommended concentration: 0.2-0.5ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Skeletal Muscle and Kidney. Recommended concentration: 2ug/ml.
Background References:
  • McDuffie M, Maybee NA, Keller SR, Stevens BK, Garmey JC, Morris MA, Kropf E, Rival C, Ma K, Carter JD, Tersey SA, Nunemaker CS, Nadler JL. Nonobese diabetic (NOD) mice congenic for a targeted deletion of 12/15-lipoxygenase are protected from autoimmune diabetes. Diabetes. 2008 Jan;57(1):199-208. Epub 2007 Oct 16.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-HYKTDVAVKDDPE
NCBI Gene ID #:
246
NCBI Official Name:
arachidonate 15-lipoxygenase
NCBI Official Symbol:
ALOX15
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_001131.3
Protein GI Number:
40316937
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Obesity
SPECIFICITY:
.
Swissprot #:
P16050