ALS2CR2 Antibody

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ProSci
Product Code: PSI-45-246
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-246-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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HEK293 overexpressing ILPIP and probed with 45-246 (mock transfection in first lane).
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45-246 Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and cytoplasmic staining. The nuclear stai
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HEK293 overexpressing ILPIP and probed with 45-246 (mock transfection in first lane).
45-246 Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and cytoplasmic staining. The nuclear stai

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Further Information

Additional Names:
STRADB, ALS2CR2, ILPIP, PAPK, ILPIPA, CALS-21, PRO1038, amyotrophic lateral sclerosis 2 (juvenile) chromosome region, candidate 2, ILP-interacting protein ILPIPA, STE20-related kinase adaptor beta, STRAD beta, pseudokinase ALS2CR2
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 35kDa band observed in Human Heart, Liver and Muscle lysates (calculated MW of 47.0kDa according to NP_061041.2 (alpha isoform) and 31.2kDa according to AAF71042.1 (beta isoform). In transfected HEK293 transiently expressing ILPIP a band of approx. 48kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 1-3ug/ml.Immunohistochemistry:In paraffin embedded Human Heart shows staining of myocardial fibres in transverse section. Recommended concentration: 3-5ug/ml.Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasm of U251 cells. Recommended concentration: 10ug/ml.
Background References:
  • Sanna MG, da Silva Correia J, Luo Y, Chuang B, Paulson LM, Nguyen B, Deveraux QL, Ulevitch RJ. ILPIP, a novel anti-apoptotic protein that enhances XIAP-mediated activation of JNK1 and protection against apoptosis. J Biol Chem. 2002 Aug 23;277(34):30454-62.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Dog, Cow
Immunogen:
The immunogen for this antibody is: CDFPDEKDSYWEF
NCBI Gene ID #:
55437
NCBI Official Name:
STE20-related kinase adaptor beta
NCBI Official Symbol:
STRADB
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_061041.2, AAF71042.1
Protein GI Number:
13027388
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
This is expected to recognise both human isoforms: ILPIP-alpha (NP_061041.2) and ILPIP-beta (AAF71042.1).
Swissprot #:
Q9C0K7