BRD4 Antibody

ProSci
Product Code: PSI-29-005
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-29-005-100ul100ul£662.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Canine (dog)
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
For short periods of storage (days) store at 4˚C. For longer periods of storage, store BRD4 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.

Images

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Antibody used in WB on Human Thymus at 0.2-1 ug/ml.
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Antibody used in WB on Human Thymus at 0.2-1 ug/ml.

Further Information

Additional Names:
BRD4, CAP, HUNKI, MCAP, HUNK1
Application Note:
BRD4 antibody can be used for detection of BRD4 by ELISA at 1:1562500. BRD4 antibody can be used for detection of BRD4 by western blot at 1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Background:
BRD4 is homologous to the murine protein MCAP, which associates with chromosomes during mitosis, and to the human RING3 protein, a serine/threonine kinase. Each of these proteins contains two bromodomains, a conserved sequence motif which may be involved in chromatin targeting.The protein encoded by this gene is homologous to the murine protein MCAP, which associates with chromosomes during mitosis, and to the human RING3 protein, a serine/threonine kinase. Each of these proteins contains two bromodomains, a conserved sequence motif which may be involved in chromatin targeting. This gene has been implicated as the chromosome 19 target of translocation t (15;19) (q13;p13.1), which defines an upper respiratory tract carcinoma in young people. Two alternatively spliced transcript variants have been described.
Background References:
  • Schweiger, M.R., (2006) J. Virol. 80 (9), 4276-4285.
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human BRD4.
NCBI Gene ID #:
23476
NCBI Official Name:
bromodomain containing 4
NCBI Official Symbol:
BRD4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
80 kDa, 152 kDa
Protein Accession #:
NP_055114
Protein GI Number:
7657218
Purification:
Antibody is purified by peptide affinity chromatography method.
Research Area:
Transcription
Swissprot #:
O60885-2
User NOte:
Optimal dilutions for each application to be determined by the researcher.