CASP3 Antibody
| Code | Size | Price |
|---|
| PSI-31-001-100ul | 100ul | £609.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
For short periods of storage (days) store at 4˚C. For longer periods of storage, store CASP3 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Documents
Further Information
Additional Names:
CASP3, CPP32, SCA-1, CPP32B
Application Note:
CASP3 antibody can be used for detection of CASP3 by ELISA at 1:62500. CASP3 antibody can be used for detection of CASP3 by western blot at 5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Background:
CASP3 is a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein.
Background References:
- Selvakumar, P., et al., (2006) FEBS Lett. 580 (8), 2021-2026.
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CASP3.
NCBI Gene ID #:
836
NCBI Official Name:
caspase 3, apoptosis-related cysteine peptidase
NCBI Official Symbol:
CASP3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
32 kDa, 32 kDa
Protein Accession #:
NP_004337
Protein GI Number:
14790119
Purification:
Antibody is purified by protein A chromatography method.
Research Area:
Cancer ,Apoptosis
Swissprot #:
P42574
User NOte:
Optimal dilutions for each application to be determined by the researcher.