CDC25B Antibody
| Code | Size | Price |
|---|
| PSI-31-038-100ul | 100ul | £662.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Storage:
For short periods of storage (days) store at 4˚C. For longer periods of storage, store CDC25B antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Documents
Further Information
Additional Names:
CDC25B, CDC25HU2
Application Note:
CDC25B antibody can be used for detection of CDC25B by ELISA at 1:62500. CDC25B antibody can be used for detection of CDC25B by western blot at 1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Background:
CDC25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined.
Background References:
- Uchida, S., et al., (2004) Biochem. Biophys. Res. Commun. 316 (1), 226-232.
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CDC25B.
NCBI Gene ID #:
994
NCBI Official Name:
cell division cycle 25B
NCBI Official Symbol:
CDC25B
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
65 kDa
Protein Accession #:
NP_068659
Protein GI Number:
11641413
Purification:
Antibody is purified by peptide affinity chromatography method.
Research Area:
Cell Cycle ,Cancer
Swissprot #:
P30305
User NOte:
Optimal dilutions for each application to be determined by the researcher.